Publications and Presentations
miR393s regulate salt stress response pathway
miR393s regulate salt stress response pathway in Arabidopsis thaliana through scaffold protein RACK1A mediated ABA signaling pathways
Here we show that genetic loss of RACK1A- the predominant member of the three genes family of RACK1 in Arabidopsis, results in the inhibition of miR393 level causing the same salt sensitivities as the individual mir393a or mir393b or the double mutant mir393ab phenotypes. We propose that down-regulation of auxin signaling through RACK1A induced miR393 biogenesis potentially regulates the Arabidopsis acclimation to salinity. Our findings fill up a molecular gap in our understanding of the role of miR393 mediated ABA and auxin-regulated salt stress responses.
Host targeted antiviral (HTA)
Host targeted antiviral (HTA): functional inhibitor compounds of scaffold protein RACK1 inhibit HSV
Here we provide evidence that the drugs show high efficacy in inhibition of HSV-1 proliferation in a HEp-2 cell line. The drug showed similar efficacy as the available anti-herpes drug acyclovir and showed supralinear effect when applied in a combinatorial manner. As an increasing number of viruses are reported to use host RACK1 proteins, and more than 100 diverse animals and plant disease-causing viruses are known to use IRES-based translation, these drugs can be established as host-targeted broad antiviral drugs.
Optogenetics
Optogenetics: A Cellular Photoactivation Method and Its Applications in Biomedical Sciences
This review identifies optogenetics, the cascade of events that leads to alternating ion movement, and factors that influence microbial opsins protein function. It discusses factors needed to be considered for an application of optogenetics as a tool and the wavelength of light required to induce retinal isomerization on each type of opsin proteins that leads to photoactivation. Understanding the architecture of opsin proteins is critical in understanding the mechanism by which it transports individual ions.
Genomic Imprinting
Genomic Imprinting: Comparative Analysis Between Plants and Mammals
The elucidation of the genetic basis and molecular mechanisms responsible for genetic imprinting have provided answers to various crucial questions arising in biological sciences.
Editorial: Signaling through WD-Repeat Proteins in Plants
Editorial: Signaling through WD-Repeat Proteins in Plants
Tyrosine Phosphorylation Based Homo-dimerization
Tyrosine Phosphorylation Based Homo-dimerization of Arabidopsis RACK1A Proteins Regulates Oxidative Stress Signaling Pathways in Yeast
Here we show evidence that RACK1A proteins, depending on diverse environmental stresses, are tyrosine phosphorylated. Utilizing site-directed mutagenesis of key tyrosine residues, it is found that tyrosine phosphorylation can potentially dictate the homo-dimerization of RACK1A proteins.
The Receptor for Activated C Kinase in Plant Signaling
The Receptor for Activated C Kinase in Plant Signaling: Tale of a Promiscuous Little Molecule
In this review, we take a critical look at the novel information regarding the many functions in which plant RACK1 has been reported to participate, with a special emphasis on the information on its currently identified and missing ligand partners.
The Receptor for Activated C Kinase in Plant Signaling
The Receptor for Activated C Kinase in Plant Signaling: Tale of a Promiscuous Little Molecule
Two decades after the first report of the plant homolog of the Receptor for Activated C Kinase 1 (RACK1) in cultured tobacco BY2 cells, a significant advancement has been made in the elucidation of its cellular and molecular role. The protein is now implicated in many biological functions including protein translation, multiple hormonal responses, developmental processes, pathogen infection resistance, environmental stress responses, and miRNA production.
Welcome to the Brave New World
Welcome to the Brave New World: CRISPR mediated Genome Editing- pathway to designer babies?
Arabidopsis scaffold protein RACK1A interacts with diverse environmental stress and photosynthesis related proteins
Arabidopsis scaffold protein RACK1A interacts with diverse environmental stress and photosynthesis related proteins
Here we present the use of Arabidopsis RACK1A, the predominant isoform of a 3-member family, as a bait to screen a split-ubiquitin based cDNA library.
Arabidopsis scaffold protein RACK1A modulates rare sugar D-allose regulated gibberellin signaling.
Arabidopsis scaffold protein RACK1A modulates rare sugar D-allose regulated gibberellin signaling
Using genetic knockout lines and a reporter gene, the functional role of RACK1A in the D-allose pathway was investigated. It was found that the rack1a knockout seeds showed hypersensitivity to D-allose-induced inhibition of seed germination, implicating a role for RACK1A in the D-allose mediated suppression of seed germination. On the other hand, a functional RACK1A in the background of the double knockout mutations in the other two RACK1 isoforms, rack1b/rack1c, showed significant resistance to the D-allose induced inhibition of seed germination.